Description
ROTI®Fect RNAi Kit, ready-to-use, CELLPURE®, for transfection, Storage temp. +4 °C
New, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus.ROTI®Fect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI®Fect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells.The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week.
- Optimised RNA release into the cytosol
- High levels of gene silencing with low RNA concentration
- Efficient knockdown after 48 hours already
- No elaborate optimisation necessary
- Very low cell toxicity
- Suitable for cell lines and primary cells
- Straightforward, efficient protocol for whole medium included
Primary cells: MCS, M1Cell lines: A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2
0.2 and 1 ml, respectively, ROTI®Fect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI®Fect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément.Approx. 650 assays per ml in 24wells. Dependend on the cell line used.