ROTI®Garose-Biotin Beads, for biochemistry, 5 ml, plastic
Avertissement
€564,40
Quantité
Compliance & Safety Information
Avertissement
Hazard Statements (H-Phrases)
H226
Flammable liquid and vapour
H319
Causes serious eye irritation
Precautionary Statements (P-Phrases)
P210
Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280
Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
ROTI®Garose-Biotin Beads, for biochemistry, Density (D) 0,85 to 0,95 g/cm³, Flash point (flp) >50 °C, Boiling point (bp) >80 °C, Storage temp. +4 °C, UN-Nr. 1170
ROTI®Garose-Biotin and Streptavidin beads offer the perfect solution for the isolation of biotinylated and streptavidinated molecules in benchtop column chromatography or in batch procedures.
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of biotinylatd or avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
Directions for use
The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
Biotin coupled agarose beads for affinity chromatography.50 % bead suspension in water stabilised with 0,02 % sodium azide.
- One-step isolation of avidin/streptavidin-tagged molecules from total lysates
- For batch mode and gravity flow
Suitable for
Recommended for isolation of avidin- or streptavidin labelled molecules in batch mode or via columns via low pressure affinity chromatography.
Directions for use
The matrix of ROTI®Garose-Biotin Beads consists of beaded 4 % cross-linked agarose.Biotin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing biotin leakage during elution, but also enhancing the binding capacity by reduction of steric effects. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
Technical Information
| Instructions for use, addition | Bead suspension, binding capacity >30 mgl/ml packed matrix |
0844.1
ROTI®Garose-Biotin Beads, for biochemistry, 5 ml, plastic
5 ml / 1 piece
€564,40