Description
Size exclusion chromatography columns, ROTI®Dex-25 Medium Grav L, Storage temp. +2 to +25 °C
Prepacked ROTI®Dex-Grav columns for size exclusion chromatography by gravity, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
The column bed consists of ROTI®Dex-25 Medium (Art. No. 21A5), pre-swollen in deionised water.
- Oligonucleotides > 10 bp
- Globular proteins > 5 kDa
- Particles > 2 nm
Column size L is recommended for sample volumes of 2.5 ml.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Version | Grav L |
| Matrix | Cross-linked dextran |
| MWCO | 5 kDa10 bp |
| Particle size | Dry: 50-150 µmWet: 85-260 µm |
| Storage buffer | Deionized water with 0.15% ProClin® 150 |
| Sample volume | 2.5 ml |
| Elution volume | 3.5 ml |