Description
ROTI®Mark TRICOLOR, Protein marker, prestained, ready-to-use, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. -20 °C
Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel.
Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker.
Protein ladder including tricolour-prestained proteins of 10 up to 245 kDa. In order to visualise separation during the gel run and for better orientation in the gel, two proteins of approx. 25 and 75 kDa have been prestained in green and red, respectively, in addition to the blue coloured bands. All proteins excel by having a very even running behaviour and form distinct bands.ROTI®Mark TRICOLOR is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot.
Fig.: 4-20 % gradient SDS-PAGE (Tris)
| Number of protein bands | 12 |
| Amount of traces | ~200/ml |
| Colour | blue prestained |
| Size range | 10-245 kDa |
| Detection in western blot | no detection |
| Quantification | non recommendé |
| Reference bands | 25 kD green, 75 kD red |
| Dispatch | ready-to-use |