Beschrijving
anti-keratine type II muis, monoklonaal, Ks pan1-8, 50 µg/ml, affiniteit-gezuiverd (geproduceerd door PROGEN), dichtheid (D) ~1 g/cm³, kookpunt (bp) ~100 °C, opslagtemp. +4 °C
Dit antilichaamportfolio bevat een breed scala aan weefsel- & tumorspecifieke primaire antilichamen voor fundamenteel onderzoek, productontwikkeling en de analyse van diverse biologische vraagstukken. De monoklonale antilichamen zijn uitstekend toepasbaar voor alle cel- of weefselbiologische studies en voor de analyse van predictieve markers in onderzoek.
- Hoge epitoopaffiniteit
- Zeer specifieke detectie van het doelwit-eiwit
- Niet-geconjugeerd
- Geschikt voor bijv. western blot, immunoprecipitatie, ELISA & immunofluorescentie
Ks pan1-8 vormt een uitstekende marker voor het onderscheiden van carcinomen van niet-epitheeltumoren. Reagerende polypeptiden: Mr 52.500-Mr 68.000 keratinen (type II keratinen K1-K8; voorheen ook aangeduid als cytokeratinen 1-8) van menselijke epitheelcellen. Specifiek gedetecteerde tumoren: alle neoplasmen van epitheliale oorsprong. Reactiviteit op gekweekte cellijnen MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.
Immunocytochemie (ICC): Assay-afhankelijkImmunohistochemie (IHC) - bevroren: 1:10-1:100 (0,5-5 μg/ml)Immunohistochemie (IHC) - paraffine: 1:10-1:100 (0,5-5 μg/ml, magnetronbehandeling aanbevolen)Western Blot (WB): 1:500 (0,1 μg/ml)
| Toepassing | ICC/IF IHC WB |
| Gastheersoort | muis |
| Klonale status | monoklonaal |
| Reactiviteit antilichaam | muis mens |
| Zuivering | Affiniteitschromatografie |
| Conjugaat | niet-geconjugeerd |
| Concentratie | 50 µg/ml |
| Isotype | IgG2a |
| Kloon | Ks pan1-8 |
| Doelwit | Cytoskeleteiwitten uit gekweekte menselijke MCF-7-cellen |
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Questions about anti-Keratine Type II muis, monoklonaal, Ks pan1-8, 0,2 ml, kunststof
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anti-Keratine Type II muis, monoklonaal, Ks pan1-8, 0,2 ml, kunststof is the article on this page. Luminix Health distributes PROGEN for laboratory use. It sits in Primary antibodies, inside Antibodies & ELISA kits, in Microbiologische media.
A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.
An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.
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