Beschrijving
ROTI®Cell DMEM, F12, steriel, met glutamine, pyruvaat en HEPES, klaar voor gebruik, CELLPURE®, dichtheid (D) ~1 g/cm³, kookpunt (kp) ~100 °C, opslagtemp. +2 tot +8 °C
DMEM : F12 is een veelgebruikt basismedium voor diverse zoogdiercellen. Het is een 1:1-mengsel van Ham's F12 en DMEM. Op deze manier wordt de rijkdom aan vitaminen en sporenelementen van Ham's F12 gecombineerd met de hoge voedingsstofconcentratie van DMEM. DMEM : F12 vormt tegenwoordig de basis voor veel serumvrije media.
Bij werken zonder FBS moeten ook groeifactoren, hormonen en lipiden, die essentieel zijn voor celgroei, aan het medium worden toegevoegd.
Deze modificatie van DMEM : F12 bevat L-glutamine, pyruvaat en 15 mM HEPES.
| Gebruik | Celkweek |
| Gebruiksinstructies, toevoeging | Beschermd tegen licht bewaren bij +2 °C tot +8 °C. Eenmaal geopend binnen 6-8 weken gebruiken. |
| Aanvullende info | De gedetailleerde samenstelling is te vinden in de specificatie. |
Recent bekeken
Misschien vind je dit ook leuk
Browse related categories
Category
Sub-category
Biological buffers (Tris, HEPES, MOPS)
Catalogue
Brand
Questions about ROTI®Cell DMEM, F12, steriel, met glutamine, pyruvaat en, 500 ml, kunststof
What is this article?
ROTI®Cell DMEM, F12, steriel, met glutamine, pyruvaat en, 500 ml, kunststof is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Chemicaliën.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Chemicaliën.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.