Beschrijving
ROTI®ProBlot NC Blotting Sandwich is een nitrocellulose blotting-sandwich voor de laboratoriumoverdracht van nucleïnezuren in Southern en Northern blot-toepassingen. Elke sandwich meet 70 × 84 mm en heeft een poriegrootte van 0,45 µm, wat aansluit bij compacte gelformaten en protocollen die standaard NC-porositeit vereisen. De eenheidsverpakking bevat 20 stuks en weegt 0,704 kg, wat een praktische voorraad biedt voor herhaalde blotting-runs. Deze ROTI®ProBlot sandwich van fabrikant Carl ROTH is bedoeld voor moleculair-biologische workflows waarbij nitrocellulosemembranen worden gebruikt voor de immobilisatie van nucleïnezuren na elektroforetische scheiding. Vooraf geassembleerde, voorgesneden stukken vereenvoudigen het laden van de cassette en helpen de blot-geometrie consistent te houden tussen experimenten. De poriegrootte van 0,45 µm is geschikt voor vele routinematige methoden voor de overdracht van nucleïnezuren die retentie in evenwicht brengen met de doorstroming van buffer door het membraan. Gebruik bij Southern en Northern blotting en gerelateerde membraanoverdrachtprocedures die 70 × 84 mm nitrocellulose sandwiches van 0,45 µm vereisen.
Dit artikel hoort bij Blotting-membranen & -papieren, in Proteïnebiologie & Western blot.
Belangrijkste kenmerken
- Verpakkingsgrootte: 20 stuks
- Producttype: NC blotting-sandwich
- Materiaal: Nitrocellulose
- Poriegrootte: 0,45 µm
- Afmetingen: 70 × 84 mm
- Technisch informatieblad:
- Veiligheidsinformatieblad:
Compliance & Safety Information
Gevaar
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Poriëngrootte 0.45 µm
What is this article?
ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Poriëngrootte 0.45 µm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Media.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Media.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
What should I check before I order?
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