anti-Keratin K18 mouse, monoclonal, Ks18.04, 0.2 ml, plastic

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anti-Keratin K₁₈ mouse, monoclonal, Ks₁₈.04, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45,000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Tested cultured cell lines: MCF-7.

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:20-1:200Immunohistochemistry (IHC) - paraffin: 1:20-1:200 (microwave treatment recommended)Western Blot (WB): 1:100-1:500 (0.5 μg/ml-0.1 μg/ml)

Western blot analysis of human MCF-7 cell lysate with anti-Keratin K18 antibody. Western blot analysis was performed on 10 μg or 5 μg of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K18 mouse monoclonal, Ks18.04 (Cat. No. 690028) was diluted in blocking buffer (antibody concentration 0.25 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	cattle dog
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG1
Uniprot ID AK	A1XEA5 (Bovine),P05783 (human), P05784 (mouse), F1SGG1 (Pig),Q5BJY9 (rat), W5Q5M3 (sheep), W5Q5M3 (sheep)
Clone	Ks18.04
Target	Human keratin K18

Technical Documentation (PDF)

anti-Keratin K18 mouse, monoclonal, Ks18.04, 0.2 ml, plastic

Description