ROTI®ZOL RNA, ready-to-use, for molecular biology, 100 ml, glass

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ROTI®ZOL RNA, ready-to-use, for molecular biology, Density (D) 1 g/cm³, Storage temp. +4 °C, UN-Nr. 1760

ROTI^®ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue.ROTI^®ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi (Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI^®ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process.

Easy and very rapid two-step RNA isolation
High recovery rates of intact, very pure total RNA
Applicable on a broad range of tissues and on cell types
The purified RNA is ready for use in standard downstream applications such as RT-PCR

Typical RNA yield from 5x10⁶ cells: 30-35 μgTypical purity of RNA prepared from 5x10⁶ cells: 2,08 (A₂₆₀/A₂₈₀)

Figures 1 and 2:Isolation of totalRNA by ROTI^®Zol RNA.Left: Tube after centrifugation. A: Aqueous phase - RNA containig, P: Phenolic phase - protein containing. DNA accumulates in the A/P interphase.Right: Typical gel of total RNA isolated by ROTI^®Zol RNA (extracted from 5x10⁶ HL-60 cells).

Technical Documentation (PDF)