ROTIPHORESE® 5x TBE Buffer, 5x conc. buffer concentrate, 1 l, glass
€32,15
Catalog nr.: 1L9K.1
Quantity
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
H360FD
H360FD – No description available
Precautionary Statements (P-Phrases)
P280
Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313
IF exposed or concerned: Get medical advice/attention.
ROTIPHORESE® 5x TBE Buffer, 5x conc. buffer concentrate, Density (D) 1,031 g/cm³, Boiling point (bp) 100 °C, Storage temp. +15 to +25 °C
The TBE buffer is optimally suited as a gel and running buffer system.
Mechanism
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
ROTIPHORESE®5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.
Directions for use
ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
Mechanism
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE® 5x TBE Buffer, 5x conc. buffer concentrate, 1 l, glass
1 liter / 1 piece
€32,15