anti-p53 Protein mouse, monoclonal, Bp53.11, 0.2 ml, plastic

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anti-p₅₃ Protein mouse, monoclonal, Bp₅₃.11, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31. The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma. Reactivity on cultured cell lines: Hela, MCF-7.

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:1,000-1:3,000 (17-50 ng/ml)Immunohistochemistry (IHC) - paraffin: 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)Western Blot (WB): 1:2,000-1:10,000 (5-25 ng/ml)

p53 staining in human squamous cell carcinoma (image courtesy of J. Heß, University Hospital Heidelberg)

Western blot analysis of HEK lysate with anti-p53 antibody. Western blot analysis was performed on 20 μg HEK lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-p53, mouse monoclonal, Bp53.11 (Cat. No. 690039) was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	human
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG2a
Uniprot ID AK	Q96A56 (human)
Clone	Bp53.11
Target	Recombinant human p53 (transcription domain within the NH2 terminus)

Technical Documentation (PDF)

anti-p53 Protein mouse, monoclonal, Bp53.11, 0.2 ml, plastic

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