Beschreibung
HPLC-kolom NUCLEODUR®, 300-5 C4 ec, 250x4 mm
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Wijdporige RP-fase voor dagelijkse routineanalyses. De butyl-modificatie met gemiddelde dichtheid is gebaseerd op NUCLEODUR®-silicagel van hoge zuiverheid en wordt vaak gebruikt voor de scheiding van biomoleculen. Butyl (C4)-modificaties hebben een lagere retentie voor hydrofobe verbindingen in vergelijking met C18 (octadecylsilaan, ODS) of C8 (octyl, MOS)-modificaties.
| Deeltjesgrootte | 5 µm |
| Poriegrootte | 300 Å |
| L x Ø intern (kolom) | 250 x 4 mm |
| Kolomvolume | 3,15 ml |
| Endcapped | ja |
| Eluent in kolom | Acetonitril - Water |
| Fase | C4 ec |
| Oppervlaktechemie | Monomere butyl (C4)-wijdporiemodificatie met gemiddelde dichtheid |
| USP-classificatie | L26 |
| Scheidingsprincipe | Reversed phase (RP) |
| Kolomvorm | Analytische scheidingskolom |
| Kolomtype | HPLC-kolom |
| Deeltjestype | Volledig poreuze deeltjes (FPP) |
| Specifiek oppervlak volgens BET | 100 m²/g |
| Porievolume | 0,9 ml/g |
| Temperatuurstabiliteit | 60 ° C |
| Interacties / retentiemechanisme | hydrofoob (van der Waals-interacties), enige resterende silanol-interacties |
| Drukvastheid | 450 bar |
| pH-stabiliteit | 1,0-9,0 |
| Aanbevolen toepassing(en) | Biomoleculaire macromoleculen, zoals eiwitten en peptiden |
| Soortgelijke fasen | Jupiter® C4 ACE® C4 |
| Applicatiedatabase (Macherey-Nagel) | HPLC |
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Questions about HPLC-kolom NUCLEODUR®, 300-5 C4 ec, 250x4 mm, 1 eenheid
What is this article?
HPLC-kolom NUCLEODUR®, 300-5 C4 ec, 250x4 mm, 1 eenheid is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatographie.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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