Beschreibung
Ligase T4, 5 U/µl, for Molecular Biology, Density (D) 1,18 g/cm³, Storage temp. -20 °C
ATP-dependent recombinant ligase for molecular cloning, site-directed mutagenesis, pitch repair in duplex DNA, RNA or DNA/RNA hybrids and ligation-mediated PCR.
- Cloning of PCR and restriction fragments
- Self-circulation of linear DNA
- Site-directed mutagenesis
T4 DNA Ligase catalyses the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl end groups in duplex DNA or RNA.
The kit contains:
T4 DNA LigaseLigation buffer (10x)ATP solution (25 mM)The individual components of this kit cannot be purchased separately.The tubes included in the kit are colour coded to avoid confusion of reagents.