Beschreibung
Nile blue A (C.I. 51180), 80 %, Molar mass (M) 732,84 g/mol, CAS No. 3625-57-8, EC No. 222-832-5, Empirical formula C40H40N6O6S
Lipid staining dye, soluble in water (acidulated with H2SO4). It stains neutral lipids pink and acid lipids blue.
Technical Information
| Application | saturated aqueous solution (acidulated with H2SO4) |
| Dyeing | dead cells blue |
| Absorption maximum | 633 nm (in 50% EtOH) |
| Colour conversion | blue - violet-red |
| Soluble in | Fat/oil |
| Detection | neutral lipids pink, acid lipids blue |
| Ox. colour | blue-red |
| Transition range pH | 10,2 - 13,0 |
| Red. colour | colourless |
| Redox potential E0[V] at pH=0 and 20 °C | +0.41 |
| Use | vitality test |