Beschreibung
Rhodamine-12-dUTP, min. 95 %, 1 mM oplossing, Molaire massa (M) 990,7 g/mol, Dichtheid (D) 1 g/cm³, Kookpunt (kp) 100 °C, Opslagtemp. -20 °C, Brutoformule C39H41N6O19P3
- Vrij van DNAse, RNAse en protease
- Vrij van PCR-remmers zoals gemodificeerde basen en tetra-pyrofosfaat
- Ingesteld op pH 7,5 voor optimale enzymcompatibiliteit
- Zeer efficiënte enzymatische productie
- Optimaal te combineren met Carl ROTH ROTI®Pol DNA-polymerasen
Alle nucleotiden van Carl ROTH worden vervaardigd uit reagentia van de hoogste kwaliteit en zijn uitvoerig getest op kwaliteit. Deze testprocedure omvat niet alleen standaard-PCR, maar ook "long range PCR", herhaalde kwantitatieve light-cycling-reacties en tests voor fysische stabiliteit.
Rhodamine-12-dUTP kan dTTP vervangen in groeiende DNA-strengen en wordt gebruikt voor efficiënte niet-radioactieve DNA-labeling. Detectie van gelabelde nucleïnezuren kan eenvoudig plaatsvinden door directe analyse van fluorescente signalen (bijv. door fluorescentiemicroscopie). Rhodamine-12-dUTP kan ook worden gebruikt voor dubbelkleuringstechnieken in combinatie met fluoresceïne-12-dUTP of biotine-11-dUTP en de respectievelijke antilichamen of streptavidine-complexen.Excitatie: 505 nmEmissie: 530 nm (rood)
Niet-radioactieve labeling van DNA via enzymatische reacties, bijv. PCR, reverse transcriptie, nick-end-translation, end-labeling of random-primed DNA-labeling. Inbouw kan plaatsvinden met alle gevestigde DNA-polymerasen (bijv. Taq-polymerase, T4 DNA-polymerase, Klenow-fragment). Getest op de afwezigheid van endo-, exodeoxyribonuclease, ribonuclease en fosfatase.
Rhodamine Green, mengsel van 5/6 isomeren ε505 (pH 7) = 8,5 E x mmol-1 x cm-1; pH: 7,5 ±0,2
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Questions about Rhodamine-12-dUTP, min. 95 %, 1 mM oplossing, 25 µl, plastic
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Rhodamine-12-dUTP, min. 95 %, 1 mM oplossing, 25 µl, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Fluorescent dyes & labels, inside Dyes, stains & indicators, in Labor- und Industriechemikalien.
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