Beschreibung
ROTI®Garose-Protein A/G HPBeads, for biochemistry, Density (D) 0,85 to 0,95 g/cm³, Flash point (flp) >50 °C, Boiling point (bp) >80 °C, Storage temp. +4 °C, UN-Nr. 1170
For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
- Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
- High yield of very pure immunoglobulins
- Easy elution and regeneration
- Covalent binding of protein G in high ligand density
The matrix of ROTI®Garose-Protein A and G Beads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.The recombinant protein A (recA) shares IgG binding properties with natural protein A of S. aureus Cowan strain I. It has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM.Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity. It has high affinity for IgG from a variety of different mammalian species, also binding IgG3 with high affinity.
Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0). ROTI®Garose-Protein A and G Beads may be regenerated, making them very cost-effective.May not be autoclaved.
Mixture (1:1) of protein A- and protein G coated agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. High-performance affinity resin for antibody purification.In ROTI®Garose-Protein A/G HPBeads, the advantages of the highly efficient protein A and G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.50 % bead suspension in 20 % ethanol.
- Binding capacity (human IgG) of approx. 25 mg per ml matrix
- Superior affinity for a wide range of IgGs
- Binding of some populations IgA and IgM
- Recommended for the isolation of rat IgGs
- For batch mode, gravity flow, MPLC und FPLC
- Recommended for big matrix volumes
All small-scale applications, for MPLC and FPLC, or if antibodies shall be isolated either in short time or in big amounts.
The matrix of ROTI®Garose-Protein A/G HPBeads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.High binding affinity to IgGs of many different mammalian species, high affinity binding of all IgG3, binding of some populations IgA and IgM.
| Instructions for use, addition | Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) |
Compliance & Safety Information
Warnung
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)