ROTI®Pol ProofRead (1 x 40 µl), 5 U/µl, 40 µl, plastic

For PCR. Recombinant, heat stable DNA polymerase from the thermophilic bacterium Thermus aquaticus or Pyrococcus furiosus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Recombinant version of the heat stable Pfu DNA polymerase from the extreme thermophilic archae bacterium Pyrococcus furiosus in storage buffer, plus additional 10x concentrated PCR-ProofRead reaction buffer. ROTI^®Pol ProofRead is recommended for use in all PCR applications in which exact sequence fidelity is required.This polymerase set ROTI^®Pol ProofRead is the optimal choice for all high fidelity PCR assays, as being performed, for instance, prior to cloning or sequencing, in cycle sequencing or in site-directed mutagenesis reactions. In combination with the attached specially adjusted buffer, the ProofRead polymerase delivers sequence identical PCR amplificates of good yield with a wide range of PCR templates. Additionally, ROTI^®Pol ProofRead is well suited for amplification of target sequences of high GC content, or with complex secondary structures.ROTI^®Pol ProofRead is able to amplify PCR products up to 3 kb with genomic DNA and is appropriate for use in the amplification of a broad variety of template DNAs. The ProofRead DNA polymerase included in the set replicates DNA 5’ → 3’ at 72 °C to 75 °C under presence of magnesium ions. Furthermore, the ProofRead-DNA polymerase possesses a 3’ → 5’ (proof reading) exonuclease activity, rapidly substituting misincorporated bases during polymerization, and thus being responsible for the high sequence fidelity. ROTI^®Pol ProofRead generated DNA fragments are blunt-ended.

Filled in colour coded tubes, the set contains the DNA polymerase and a 10x concentrated proof-reading reaction buffer with 20 mM MgSO₄.

ProofRead polymerase in storage buffer containing 50 % glycerol, PCR ProofRead buffer (10x) with 20 mM MgSO₄. Colour coded tubes.Contents of this set may not be bought separately.