Beschreibung
ROTI®Pol TaqUltra (1 x 40 µl), 5 U/µl, DNA-free, Density (D) ~1 g/cm³, Storage temp. -20 °C
For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.
ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.
Recombinant full-length form of the heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer and tested on the absence of bacterial DNA. ROTI®Pol TaqUltra is recommended for use in PCR applications in general, or if bacterial DNA, or in RT-PCR, bacterial 16S rRNA shall be detected. ROTI®Pol TaqUltra is purified using a multiple-step process that minimizes contaminating bacterial DNA to a none detectable level. Each lot of the polymerase undergoes strict quality control testing in order to ensure the absence of detectable amounts of contaminating bacterial DNA. ROTI®Pol TaqUltra is provided in a DNA-free storage buffer containing 50 % glycerol.ROTI®Pol TaqUltra is able to amplify PCR products up to 3 kb and is appropriate for use in the amplification of DNA from eukaryotic as well as prokaryotic templates. The TaqUltra DNA polymerase possesses a 5' → 3' polymerase- as well as a 5'-3' exonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.
| Application | PCR of bacterial gDNA |
| Amplicon ends | 3'dA |
| Polymerase | DNA free, ultra pure Taq polymerase |
| Use | PCR of bacterial gDNA |
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Questions about ROTI®Pol TaqUltra (1 x 40 µl), 5 U/µl, DNA-free, 40 µl, plastic
What is this article?
ROTI®Pol TaqUltra (1 x 40 µl), 5 U/µl, DNA-free, 40 µl, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in PCR, qPCR & nucleic acid analysis reagents, inside Molecular biology, in Mikrobiologie.
PCR and qPCR reagents are the polymerase, the nucleotides, the buffer and often a ready master mix, plus the dyes a real-time instrument reads. Hot-start enzymes and proofreading enzymes are different tools. A master mix already contains nucleotides. Adding them again is a failed reaction.
Molecular biology here is the handling of DNA and RNA. Extraction kits break the cell and then separate the nucleic acid from protein and from the other nucleic acid. PCR and qPCR reagents copy a chosen sequence so it can be seen. The enzymes and the nucleotides are what the copy depends on. Nuclease contamination destroys the sample before the kit can save it. Sequences are on NCBI Nucleotide. Papers are on PubMed.
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The category is Molecular biology. The sub-category is PCR, qPCR & nucleic acid analysis reagents. The catalogue is Mikrobiologie.
Shelves beside this one are DNA & RNA extraction kits and Molecular biology reagents (other).
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