Beschreibung
ROTI®Prep DNA Micro, 10 pieces, for molecular biology, Density (D) ~1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep DNA Micro was developed to isolate genomic DNA from small sample amounts of different source materials.The isolation is based on a new method and combines sample lysis with the subsequent binding of the DNA to the surface of a column membrane. After several washing and centrifugation steps, the DNA is eluted from the membrane using an elution buffer.
- Specially developed for DNA extraction from small sample quantities
- Preparation in the well-established mini-column system
- Fast, easy and reliable
- Extraction time of approx. 8 min (excluding lysis)
- Cell remnants or small tissue samples (up to 5 mg)
- Eucaryotic cells (up to 1x106)
- Blood samples (up to 50 µl) and Bloodsticks
- Paraffin-embedded tissue samples
Lysis Buffer LSM, Binding Buffer BSM, Proteinase K, Washing Buffer WSA (conc.), Washing Buffer WST (conc.), Elution Buffer EB, centrifugation columns, collection tubes, elution tubes, detailed instructions.Ethanol 99.8 % (p.a., Art. No. 9065), RNase free water (Art. No. 1HPE.1), 1.5 ml reaction tubes (Art. No. 1KPA.1) and RNase A (optional, Art. No. 7156.1) are not included in the kit and should be ordered separately if required.
| Basic material | Small sample quantities, cell debris/small tissue samples, eukaryotic cells, blood samples |
| Quantity of material | Cell debris (<5 mg), eukaryotic cells (up to 1x106), blood samples (< 50 µl) |
| Preparation time | approx. 8 min (excluding lysis) |
| Version | Mini prep |
| Automatic use | no |
| Elution volume | 50-100 µl |
| Typical purity (A260/A280) | 1.7-2.0 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)