Beschreibung
ROTIPHORESE® Blue R, Brillant Blue R concentrate, Density (D) 0,8 g/cm³, Flash point (flp) >10 °C, Boiling point (bp) >65 °C, UN-Nr. 3286
Concentrate for easy preparation of Coomassie® Blue R staining solutions. ROTIPHORESE®Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture.
Concentrate contains Coomassie® Blue R, methanol and pure acetic acid. ROTIPHORESE®Blue R can be diluted directly in the bottle with 400 to 600 ml deionized water, resulting in the following composition depending on the dilution.When diluted with 397,5 ml water (to a total of 1 litre staining solution): Methanol (50 %), acetic acid 10 %, Coomassie® R (0,25 %)When diluted with 507,5 ml water (to a total of 1,1 litre staining solution): Methanol (45 %), acetic acid 9 %, Coomassie® R (0,22 %)When diluted with 602,5 ml water (to a total of 1,2 litre staining solution): Methanol (41,5 %), acetic acid 8,3 %, Coomassie® R (0,2 %).
We recommend mixing the concentrated solution well before dilution and filtering the diluted solution before application.
| Sensitivity | 50 |
| Use | Coomassie® staining solution concentrate for rapid staining |