ROTIPHORESE® NF 10 x TBE Buffer, 10x conc. buffer concentrate, 2.5 l, glass
€138,70
Catalog nr.: A118.1
Menge
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
H360FD
H360FD – No description available
Precautionary Statements (P-Phrases)
P280
Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313
IF exposed or concerned: Get medical advice/attention.
ROTIPHORESE® NF 10 x TBE Buffer, 10x conc. buffer concentrate, fluorescence-free, Density (D) 1,061 g/cm³, Boiling point (bp) 100 °C, Storage temp. +15 to +25 °C
The TBE buffer is optimally suited as a gel and running buffer system.
Mechanism
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
10-times concentrated buffer formulated specially for automated sequencing. 10x stock solution contains 0,89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8,3.
Directions for use
Suitable for manufacturing sequencing gels with ROTIPHORESE®NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer.In sequencing, NF-TBE buffer is usually used in a final concentration of 1x.
Ideally suited as a gel and running buffer system and also for sequencing gels,
ROTIPHORESE® NF 10 x TBE Buffer, 10x conc. buffer concentrate, 2.5 l, glass
2.5 liter / 1 piece
€138,70