Beschreibung
SDS-PAGE sample buffer concentrated is a pharmacopoeia-quality laboratory reagent for preparing protein samples prior to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). It is specified as Reag. Ph. Eur. in accordance with European Pharmacopoeia Chapter 4.1.1 under reagent number 1115000, placing it among reagents and volumetric standard solutions of pharmacopoeial quality. The concentrated buffer is offered in a 125 ml pack size. Density is 1,04 g/cm³ and the boiling point is 100 °C, values that support dispensing, typical heating of electrophoresis samples, and laboratory records. As a concentrated SDS-PAGE sample buffer, it is combined with protein solutions so that polypeptides can be denatured and coated with detergent before loading onto polyacrylamide gels; the concentrated format allows the working ratio to be set in the laboratory. This reagent from manufacturer Carl ROTH belongs to the Ph. Eur. Reagents 4.1.1 group used in analytical and biochemical laboratories that require pharmacopoeia-referenced chemicals. The 125 ml unit, corresponding to a net weight of 0,300 kg, is suited to routine sample preparation for SDS-PAGE.

