Beschreibung
X-Gluc, min. 99 %, for microbiology, Molar mass (M) 539,81 g/mol, Storage temp. -20 °C, CAS No. 114162-64-0, Empirical formula C20H26N2O7ClBr · H2O
For colorimetric detection of glucuronidase activity. During hydrolysis of X-gluc two molecules are formed, glucuronic acid and 5-bromo-4-chloro-indoxyle, which is then being oxidized to a deep-blue indigo-dye.
X-Gluc is used for detection of contaminations by E. coli bacteria in food, waters and feeds. In molecular genetics, it is widely used as marker for expression analysis of target genes.
Technical Information
| Enzyme | β-D-Glucuronidase |
| Detection | Colour (blue) |
| Signal product | Precipitate (detection of living cells) |