ROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, plastic - Luminix Health
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ROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, plastic - Luminix Health

ROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, plastic

SKU: 25KP.1
Volume: 1 ml
Value Packs: 1 piece
Price:
€80,65

Description

ROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, Density (D) ~1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C

ROTI®Lux Probe qPCR Master Mixes are suitable for all common types of probe-based assays, for probe-based genotyping using multiplexing and for the analysis of gene expression in quantitative real-time PCR.The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (<1pg) can be detected.The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (>10x).

Properties:
  • High temperature stability
  • Excellent sensitivity (<1 pg cDNA)
  • Ultra-fast qPCR in <20 min
  • Compatible with all common probe systems (primer probes, hydrolysis probes such as TaqMan®, hybridization probes)
  • For all common qPCR cyclers - optionally with or without ROX
Applications:
  • Probe-based qPCR analyses
  • Multiplexing
  • Gene expression analyses
  • Genotyping

Master Mix without the reference dye ROX (6-Carboxy-X-rhodamine)

Suitable for:
  • Exicycler™(Bioneer)
  • CFX Real-Time PCR Cycler (Biorad)
  • RealLine Cycler (Bioron)
  • Dtlite & Dtprime Cycler (DNA Technology)
  • Mastercycler® (Eppendorf)
  • LightCycler® (Roche)
  • AriaMx Real-time PCR System (Agilent)
  • Q-Cycler (Quantabio)
Stability at room temperature: The ROTI®Lux qPCR Mastermixes can be stored at room temperature (≤30 °C) for at least 14 days.
Sensitivity and dynamic range: The ROTI®Lux Probe qPCR Master Mixes deliver consistent and reliable results, even at low input amounts such as 0.1 pg cDNA, or 1 template per reaction.
Stability by multiple freeze and thaw cycles: The stability of the ROTI®Lux qPCR master mixes is guaranteed even after multiple freeze and thaw cycles (≥10).
Robust sensitivity: The ROTI®Lux Probe qPCR Master Mixes provide stable and quantifiable results even at high Cq levels.
Not a medical device / Not an IVD product
Technical Information
Application Probe-based real-time PCR assays 
Amplicon ends 3'dA 
Basic material DNA (cDNA / gDNA) 
Polymerase HotStart DNA Polymerase 
Instructions for use, addition The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl2 and the reaction buffer. Primer and template must be added separately.1 ml Master Mix is sufficient for one 96-well plate. 
Version no ROX 

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