Description
HPLC column NUCLEODUR® 300-5, C4 ec, 5µm, 250x3mm
Material: stainless steel.
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Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
Delivery incl. 1x analytical HPLC column, instruction leaflet, certificate of analysis.
Technical Information
| Particle size | 5 µm |
| Pore size | 300 Å |
| L x Ø internal (column) | 250 x 3 mm |
| Column volume | 1.75 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | C4 ec |
| Surface chemistry | Medium density monomeric butyl (C4) wide-pore modification |
| USP listing | L26 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 100 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | hydrophobic (van der Waals interactions), some residual silanol interactions |
| Pressure stability | 450 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Biomolecular macromolecules, like proteins and peptides |
| Similar phases | Jupiter® C4 ACE® C4 |