Description
HPLC Column NUCLEODUR®, CN-RP, 3µm, 125x2mm
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The endcapped cyanopropyl phase is based on high purity NUCLEODUR® silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. The polarity can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, π-π, and also hydrophobic interactions. Therefore, this phase shows a distinct selectivity for polar organic compounds as well as for molecules containing π electron systems (e.g., analytes with double bonds). Cyanopropyl HPLC columns can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase.
| Particle size | 3 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 125 x 2 mm |
| Column volume | 0.37 ml |
| Endcapped | no |
| Eluent in column | Acetonitrile - Water |
| Phase | CN-RP |
| Surface chemistry | Cyano, nitrile (CN) modification |
| USP listing | L10 |
| Separation principle | Multi-mode (NP and RP), Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | π-π and polar (H bond), hydrophobic |
| Pressure stability | 600 bar |
| pH stability | 1,0-8,0 |
| Recommended application(s) | Molecules containing π-electron systems Organic acids Steroids Tricyclic antidepressants |