Description
Magnesium chloride solution, 25 mM, sterile, for molecular biology, Molar mass (M) 95,22 g/mol, Density (D) 1,003 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, Empirical formula MgCl2
Sterile
In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.
Application:
- Too high magnesium concentration can lead to reduced specificity and undesired PCR products
- Too low a magnesium concentration can lead to no PCR product at all
- Magnesium influences the melting point of double-stranded DNA