Description
p-Nitrotetrazolium blue chloride, min. 98 %, p.a., Molar mass (M) 817,65 g/mol, Storage temp. +4 °C, CAS No. 298-83-9, EC No. 206-067-4, Empirical formula C40H30Cl2N10O6
Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase.
Mechanism
During dimerisation of ketonized X-Phos, H+ ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
Technical Information
| Application | Western, in situ, Histochemistry |
| Enzyme | Alkaline phosphatase |
| Detection | Colour (brown-violet) |
| Signal product | Precipitate (Blot) |