ROTI®Prep Blood Genomic DNA MINI, 250 preparations, for molecular biology

Roti^®Prep Blood Genomic DNA MINI has been specially designed for the isolation of high purity genomic DNA from blood samples.The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.

The Roti^®Prep Blood Genomic DNA MINI kit has been adapted to gain high purity DNA from fresh or frozen blood (EDTA or citrate stabilised) or whole blood, respectively. In order to get optimal results, two protocols, depending on sample amount, are provided. Furthermore, the properties of the binding buffer have been optimised to reach highest binding capacity with blood samples, permitting a yield of up to 30 μg gDNA out of 400 μl blood.Usage is uncomplicated and fast to achieve (approx. 25 minutes, lysis included), and offers best conditions for following downstream applications. Isolated gDNA is free of EDTA, RNA, or proteins, and may directly be applied to all standard downstream applications like, for instance, restriction digest, PCR etc.

Lysis Buffer LSN, Binding Buffer BR, Proteinase K, Washing Solution WSO, Washing Buffer WSH, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.Contents of this Kit may not be bought separately.Please order ethanol 99,8 % (p.a., Art. No. 9065) and RNase A (optional, Art. No. 7156) separately.