Description
EC 250/4 NUCLEOSIL 100-5 Protect I is an EC HPLC separation column intended for analytical liquid chromatography. It provides a column length of 250 mm with an internal diameter of 4 mm and is delivered as one piece per pack. The NUCLEOSIL 100-5 Protect I phase designation defines the stationary-phase chemistry of this packing for HPLC separations that specify Protect I media. EC column hardware suits conventional HPLC systems used in research, quality-control, and method-development laboratories. The 250 mm × 4 mm geometry corresponds to a widely used analytical format for isocratic and gradient work. This column comes from manufacturer Macherey-Nagel and supports professional chromatography workflows that require a defined NUCLEOSIL 100-5 Protect I EC column. Individual packaging simplifies installation, spare-column stocking, and instrument setup. Net weight is 0,28 kg per unit. Choose the EC 250/4 NUCLEOSIL 100-5 Protect I model when protocols call for this length, internal diameter, and Protect I phase combination on standard HPLC equipment.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Stationary phase: NUCLEOSIL 100-5 Protect I
- Column length: 250 mm
- Internal diameter: 4 mm
- EAN: 4046681082108
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Questions about EC 250/4 NUCLEOSIL 100-5 Protect I
What is this article?
EC 250/4 NUCLEOSIL 100-5 Protect I is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
Which category should I open next?
The category is HPLC columns & accessories. The sub-category is HPLC columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

