Description
EC 250/4 NUCLEOSIL 300-5 C18 is an EC HPLC separation column for analytical liquid chromatography applications. It features a column length of 250 mm and an internal diameter of 4 mm, packed with the NUCLEOSIL 300-5 C18 stationary phase. This C18 chemistry provides strong hydrophobic retention and is widely applied in reversed-phase HPLC for the separation of non-polar and moderately polar compounds. The 4 mm internal diameter suits HPLC systems configured for narrower analytical columns and supports efficient solvent use in routine and research environments. Supplied as a single piece with a net weight of 0,28 kg, the column is ready for integration into standard chromatographic setups. Laboratories in pharmaceutical, chemical, and quality-control settings can employ this format for method development, impurity checks, and quantitative assays. The EC HPLC column design from manufacturer Macherey-Nagel delivers defined dimensions and packing identity under the NUCLEOSIL 300-5 C18 designation. Select this 250 mm × 4 mm C18 column when a compact EC geometry with classic octadecyl selectivity is required.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Stationary phase: NUCLEOSIL 300-5 C18
- Column length: 250 mm
- Internal diameter: 4 mm
- EAN: 4046681082504
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Questions about EC 250/4 NUCLEOSIL 300-5 C18
What is this article?
EC 250/4 NUCLEOSIL 300-5 C18 is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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