Description
EC 250/4.6 NUCLEODUR C18 Gravity, 5 µm is an analytical HPLC separation column for reversed-phase liquid chromatography in research and quality-control settings. The column measures 250 mm in length with an internal diameter of 4,6 mm and is packed with 5 µm NUCLEODUR C18 Gravity particles that form a hydrophobic C18 bonded phase for separating non-polar to moderately polar organic substances. This EC-format HPLC column from manufacturer Macherey-Nagel is supplied as one piece per pack and fits standard analytical HPLC systems used in pharmaceutical, chemical, and life-science laboratories. Gravity C18 chemistry supports method development, quantitative assays, and impurity profiling where stable retention behaviour and consistent peak shape are important. Typical mobile phases based on water with acetonitrile or methanol, together with suitable buffers, can be applied in line with established methods. The 250 mm bed length provides useful resolution for complex mixtures, while the widely used 4,6 mm internal diameter offers familiar flow-rate ranges, loadability, and detector response for routine analytical work. The single-column packaging enables straightforward installation and replacement in laboratories that require reliable reversed-phase HPLC performance day to day.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column type: EC HPLC separation column
- Stationary phase: NUCLEODUR C18 Gravity
- Particle size: 5 µm
- Column length: 250 mm
- EAN: 4046681005022
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Questions about EC 250/4.6 NUCLEODUR C18 Gravity, 5 µm
What is this article?
EC 250/4.6 NUCLEODUR C18 Gravity, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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