Description
EC 4/3 NUCLEODUR C18 ISIS, 5 µm is an HPLC guard column intended to shield analytical main columns during reversed-phase separations. Each cartridge is 4 mm long with a 3 mm internal diameter and contains NUCLEODUR C18 ISIS packing at 5 µm particle size, providing phase continuity with corresponding analytical columns. The product is supplied in packs of three and is suitable for main columns of 3, 4 or 4,6 mm internal diameter when used with the Column Protection System guard column holder (REF 718966). Upstream placement of the guard column reduces contamination of the analytical bed by particulates and highly retained matrix components, helping preserve efficiency, retention times and overall column durability. The NUCLEODUR C18 ISIS chemistry offers the steric selectivity associated with this specialised C18 phase, supporting robust method performance in pharmaceutical, environmental and general analytical laboratories. Available from manufacturer Macherey-Nagel, the EC 4/3 5 µm format is a straightforward consumable for laboratories that standardise on NUCLEODUR C18 ISIS 5 µm main columns and need matched guard protection in everyday HPLC operation.
This article is part of HPLC columns, in HPLC columns & accessories.
Key features
- Pack size: 3 pc(s)
- Product type: HPLC guard column
- Phase: NUCLEODUR C18 ISIS
- Particle size: 5 µm
- Dimensions: 4 mm length × 3 mm ID
- EAN: 4046681057632
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Questions about EC 4/3 NUCLEODUR C18 ISIS, 5 µm
What is this article?
EC 4/3 NUCLEODUR C18 ISIS, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC guard columns, inside HPLC columns & accessories, in Chromatography equipment.
A guard column is a short column of the same phase as the analytical column, placed in front of it to catch particles and compounds that would stick forever. It is meant to be replaced. A guard of a different phase changes the separation. The holder and the cartridge are often separate parts.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
Which category should I open next?
The category is HPLC columns & accessories. The sub-category is HPLC guard columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC columns, HPLC fittings, tubing & inline filters and HPLC accessories.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

