Description
ROTI®Garose-Glu/GST Cartridges, for biochemistry, Density (D) 0,85 to 0,95 g/cm³, Flash point (flp) >50 °C, Boiling point (bp) >80 °C, Storage temp. +4 °C, UN-Nr. 1170
Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
- Rapid, mild and highly specific purification
- Suitable for isolation of GST-peptides and protein complexes of all sizes
- High binding capacity
- Easy elution and regeneration
Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chromatography conditions.
The matrix of ROTI®Garose-Glu/GST Beads consists of beaded 4 % agarose without crosslinking, coupled with glutathione. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes. ROTI®Garose-Glu/GST Beads may be regenerated, making them very cost-effective.
Prepacked cartridges with glutathione coupled agarose beads for medium pressure affinity chromatography (MPLC).ROTI®Garose-Glu/GST Cartridges offer the perfect solution for your specific application of chromatography under medium pressure (MPLC, FPLC).Matrix in 20 % ethanol.
- Easy isolation of GST-tagged proteins from total lysates
- Recommended flow rate 0,5-5 ml/min.
- Standard ports (10-32)
- For medium pressure flow chromatography
- Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe
Optimal for isolation of glutathione-S-transferase (GST) or GST fusion proteins via automated liquid chromatography, or if proteins shall be isolated under pressure. Suitable for ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.
ROTI®Garose-Glu/GST Cartridges are prepacked and may be used directly.Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.
| Instructions for use, addition | Prepacked columns, binding capacity ≥50 mgl/column |
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)