Description
ROTI®Garose-His/Ni HPBeads plus is a biochemistry medium formulated for affinity chromatography in the laboratory. contains nickel-based high-performance beads intended for bio-chromatography, including workflows with histidine-tagged proteins. Density ranges from 0,85 to 0,95 g/cm³, the flash point is above 50 °C and the boiling point exceeds 80 °C, supporting standard handling during column packing or batch use. His/Ni bead systems are employed in immobilised metal affinity chromatography for recombinant protein isolation. The HPBeads plus designation places this resin in the high-performance segment of the ROTI®Garose range for affinity separations. Available from manufacturer Carl ROTH, the product fits molecular biology and biotechnology settings focused on protein purification. quantity is practical for optimisation trials, small-scale preparations and instructional chromatography. Application focus remains affinity chromatography within broader bio-chromatography practice that may also involve size-exclusion or hydrophobic interaction steps. Clear physical specifications help laboratories align the medium with existing purification protocols.
This article is part of Sorbents, silica gel & ion exchangers, in SPE & sample preparation.
Key features
- Particle size: 50-150 µm
- Beads (cross-linked agarose): 6 %
- Ligand: polychelator
- Metal ion: nickel
- Binding capacity (gel) (GFPuv-6xHis): ≥ 80 mg/ml
- Ethanol: 20 %
- Pack sizes: 25 ml, 100 ml
- Density: 0,85 to 0,95 g/cm³
- Flash point: >50 °C
- Boiling point: >80 °C
- Storage temperature: +4 °C
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
WarningHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Garose-His/Ni HPBeads plus, for biochemistry, 25 ml, plastic
What is this article?
ROTI®Garose-His/Ni HPBeads plus, for biochemistry, 25 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Sorbents, silica gel & ion exchangers, inside SPE & sample preparation, in Chromatography equipment.
Loose sorbent, silica gel and ion-exchange resin are the phases bought in bulk to pack a column or to stir into a solution. Particle size and activity, or the ion-exchange capacity, are the specification. A TLC silica and a flash silica of different particle sizes are not the same separation.
Solid-phase extraction holds a compound on a sorbent while the rest of the sample is washed away, then releases it in a small volume. The sorbent, the bed mass and the solvents are the method. Silica, ion exchangers and polymer phases do different jobs. A flash column is the same idea scaled toward purification rather than trace cleanup. The molecules are on PubChem, named by IUPAC.
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The category is SPE & sample preparation. The sub-category is Sorbents, silica gel & ion exchangers. The catalogue is Chromatography equipment.
Shelves beside this one are SPE cartridges & columns and Flash, empty & glass chromatography columns.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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