Description
VP 125/10 NUCLEODUR C18 ISIS, 5 µm is a VarioPrep HPLC separation column combining a 125 mm bed length with a 10 mm inner diameter and 5 µm NUCLEODUR C18 ISIS packing. This configuration supports reversed-phase liquid chromatography where intermediate length and moderate bore are preferred for resolution, loadability, and solvent use. The column incorporates an axially adjustable endfitting that permits mechanical fine-tuning of the packed bed, contributing to consistent performance under typical HPLC operating pressures. Delivered as a single unit from manufacturer Macherey-Nagel, the VP 125/10 format is appropriate for method development, semi-preparative isolation, and scale-related experiments that bridge analytical and larger preparative columns. NUCLEODUR C18 ISIS denotes a C18-modified silica stationary phase intended for hydrophobic separations of organic and biomolecular analytes. Defined length, diameter, and particle size allow straightforward matching to pumps, injectors, and detectors already configured for 10 mm ID VarioPrep hardware in pharmaceutical, chemical, and academic laboratories.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column length: 125 mm
- Inner diameter: 10 mm
- Particle size: 5 µm
- Stationary phase: NUCLEODUR C18 ISIS
- EAN: 4046681055751
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Questions about VP 125/10 NUCLEODUR C18 ISIS, 5 µm
What is this article?
VP 125/10 NUCLEODUR C18 ISIS, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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