Description
VP 250/21 NUCLEODUR C18 Gravity, 10 µm is a VarioPrep HPLC separation column built for preparative and semi-preparative reversed-phase chromatography. The column has a bed length of 250 mm and an internal diameter of 21 mm, packed with 10 µm NUCLEODUR C18 Gravity particles that provide C18 selectivity for a broad range of moderately to strongly hydrophobic analytes. An axially adjustable end fitting allows fine mechanical adjustment of the packed bed, which is useful when maintaining packing integrity under preparative flow and pressure conditions. Supplied as a single unit from manufacturer Macherey-Nagel, the column belongs to the VarioPrep family designed for flexible scale-up and fraction collection workflows. The 21 mm ID format supports higher mass load than analytical dimensions while retaining the familiar C18 Gravity chemistry used across many method-transfer scenarios. Laboratories can deploy this column for purification, isolation and larger-scale separations where robust C18 performance and adjustable end hardware are required. Dimensions, particle size and phase designation are clearly defined for straightforward matching to existing VarioPrep methods and hardware.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: VarioPrep HPLC separation column
- Stationary phase: NUCLEODUR C18 Gravity
- Particle size: 10 µm
- Length: 250 mm
- EAN: 4046681056185
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Questions about VP 250/21 NUCLEODUR C18 Gravity, 10 µm
What is this article?
VP 250/21 NUCLEODUR C18 Gravity, 10 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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