Description
ClickTech Library Kit full-length mRNA, Density (D) ~1 g/cm³, Storage temp. -20 °C, UN-Nr. 1760
The ClickTech Library Kit can be used to sequence the whole mRNA or exome of cells for genetic diagnosis. Here, an azide-modified cDNA is created from any RNA pool, which can then be highly specifically clicked with an alkyne-modified adapter sequence. The 3'-adapter-clicked cDNA can be amplified via PCR and used for further experiments.
The kit contains:
- dNTP Mix (10 mM)
- LFRS Primer RT (100 µM)
- 5x Reverse Transcription Buffer
- DTT (100 mM)
- Reverse Transcriptase (200 U/µL)
- 10x RNase H Buffer
- RNase H (5 U/µL)
- RNase A (10 mg/mL)
- 5x Azide Elongation Buffer
- 3’-N3-ddGTP (10 mM)
- Azide Elongase (20 U/µL)
- 5x Activator2
- Reactor XS
- Alkyne Adapter (100 µM)
- 2x baseclick PCR Master Mix
- LFRS Primer Forward (10 µM)
- LFRS Primer Reverse (10 µM)
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
H331
Toxic if inhaled
H350
May cause cancer
H411
Toxic to aquatic life with long lasting effects
Precautionary Statements (P-Phrases)
P201
P201 – No description available
P261
P261 – No description available
P273
P273 – No description available
P308+P313
IF exposed or concerned: Get medical advice/attention.
P391
P391 – No description available
P501
P501 – No description available