3D sample holders for light sheet microscopy

3D Sample Container for Light Sheet Microscopy is a specialised sample holder developed together with researchers for light-sheet imaging of cleared tissue pieces. External dimensions are approximately 55 × 55 × 38 mm, with a fill volume to the overflow rim of about 47 ml and an overflow-space height of roughly 10 mm. The light-entry wall is inclined about 1,9° from the vertical, tilting upward and outward. Made from optically high-grade, chemically resistant plastic with a glass-like refractive index of approximately 1,5 (Marienfeld), the container is suitable for all common aqueous and solvent-based embedding media. In light-sheet microscopy the tissue is illuminated through the side walls by a thin laser light sheet while the specimen is scanned vertically relative to the sheet; fluorescence from successive planes is recorded from above and reconstructed in 3D visualisation software. The square top view provides four numbered light-entry walls enclosing a usable sample space of about 40 × 40 × 25 mm. An upward-flared rim eases filling, shifts edge menisci outside the observation field for distortion-free imaging through the liquid surface with non-immersion dry objectives, supports secure handling and offers space for labelling or stickers. The opening accommodates immersion objectives and multi-side illumination. Containers are stackable and packed in units of 10 pieces.

This article is part of Sample containers & jars, in Bottles & containers.

Key features

• Pack size: 10 pc(s)
• External dimensions: approx. 55 × 55 × 38 mm
• Fill volume: approx. 47 ml
• Usable sample space: approx. 40 × 40 × 25 mm
• Light-entry wall inclination: approx. 1,9°
• EAN: 4064279008590