Description
5-Bromo-4-chloro-3-indolyl phosphate, p-toluidine salt,min. 99 %, BioScience-Grade, Molar mass (M) 433,63 g/mol, Storage temp. -20 °C, CAS No. 6578-06-9, EC No. 229-506-1, Empirical formula C15H15N2O4BrClP
In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in immunohistochemical procedures.
Mechanism
By enzymatic dephosphorylation BCIP is converted to indoxyl which subsequently tautomerizes to the ketone. In basic pH values, this ketone dimerizes and, in doing so, releases H+ ions. The dye precipitates in the direct vicinity of the phosphatase, hence staining this site in a weak blue colour. Enhencement and darkening of this colour is achieved by adding NBT (Art. No. 4421).
Technical Information
| Application | Western, in situ, Histochemistry |
| Enzyme | Alkaline phosphatase |
| Detection | Colour (brown) |
| Signal product | Precipitate (Blot) |
| Use | Western, in situ, histochimie |
Compliance & Safety Information
Attention
Hazard Statements (H-Phrases)
H371
May cause damage to organs
Precautionary Statements (P-Phrases)
P260
P260 – No description available
P280
Wear protective gloves/protective clothing/eye protection/face protection.
P308+P311
P308+P311 – No description available