Description
Gel Filtration Media, ROTI®Dex-50 Medium, Storage temp. +2 to +25 °C, CAS No. 9048-71-9
ROTI®Dex Medium is a spherical, porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In gel filtration, also called size exclusion chromatography, molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
ROTI®Dex enables a form of group separation for the separation of undesired, low-molecular-weight factors such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
ROTI®Dex-50 medium is excellent for the separation of proteins >25 kDa and nucleic acids >20 bp. Purified biomolecules are not significantly diluted when processed with ROTI®Dex-50 medium.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Matrix | Cross-linked dextran |
| MWCO | 25 kDa20 bp |
| Particle size | Dry: 50-150 µmWet: 100-300 µm |