Description
Inhibitor Cocktail His-tag, ready-to-use, for biochemistry, for 10 g bacteria, Density (D) ~1,1 g/cm³, Flash point (flp) 87 °C, Boiling point (bp) >180 °C, Storage temp. -20 °C
Inhibitor cocktail for inhibition of a broad range of proteases. Inhibits serine proteases, aminopeptidase B, leucine-aminopeptidases, cysteine proteases, aspartic proteases, and metalloendopeptidases.Recommended as cocktail for protease inhibition particularly for extraction of His-tagged proteins from bacterial extracts. EDTA-free to allow optimal Ni++- or Co++-his binding in affinity chromatography.
Recommended dilution approx. 1:100.Provided as stock-solution in DMSO.
Concentrations per vial are:AEBSF: 100 mMBestatin: 5 mM-64: 1.5 mMPepstatin A: 0.2 mMPhosphoamidon: 2 mM
| Recommended dilution | ~ 1:100 |
| Instructions for use, addition | 1 ml is sufficient for inhibition of proteases from 10 g bacterial cells. |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)