Description
Lambda Hind III / phiX Hae III Marker, DNA ladder + gel loading buffer, lyophil., not pre-dyed, Storage temp. -20 °C
Versatile marker for depiction of all standard DNA fragment lengths.Manufactured by restricting unmethylated DNA with the restriction endonucleases Hind III and Hae III.By mixing high molecular weight Lambda (Hind III) fragments with short-sequence phiX-174 (Hae III) fragments a marker was designed which covers a broad range from 200 bp up to 20 kb. Most fragments are distributed in an equimolar way, additionally allowing rough DNA quantification in the gel. Due to this equimolarity, however, the smallest bands of 125 bp, 118 bp and 72 bp can not be detected efficiently in standard gels.
Optimal results are obtained with a running distance of approx. 10 cm.
Fig.: 1 % agarose (NEEO, Art. No. 2267).
| Number of fragments | 17 |
| Amount of traces | ~200/100 µg |
| Size range | 72-23130 bp |
| Quantification | recommended |
| Dispatch | lyophilized |
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Questions about Lambda Hind III / phiX Hae III Marker, DNA ladder + gel loading buffer, 50 µg
What is this article?
Lambda Hind III / phiX Hae III Marker, DNA ladder + gel loading buffer, 50 µg is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiologie.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiologie.
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