Description
Phenylmethyl sulphonyl fluoride, min. 99 %, for biochemistry, Molar mass (M) 174,2 g/mol, Density (D) 0,79 g/cm³, CAS No. 329-98-6, UN-Nr. 2923, EC No. 206-350-2, Empirical formula C7H7FO2S
This article belongs on Protein biology (other), in Protein biology & Western blot.
General, irreversible inhibitor of serin proteases (chymotrypsin, trypsin and thrombin) and for the dystin-protease papain. Inhibiton by sulphonation of the serine localised in the enzymatic active site.Reversible inhibitor of cysteine-proteases. Ki = 0.91 ±0.01 mM
Working concentration: 17-174 µg/ml (0,1-1 mM).Dissolves easily in isopropanol, ethanol and methanol. Not stable in aqueous solutions (half-life value of 1 h at pH 7.5/RT).For stock solutions of 200-250 mM heat shortly (approx. 30 mins) to 30 °C.Stock solutions should be stored at -20 °C. Short term storage of few weeks may be done at +4 °C in the dark.PMSF reacts with thiols. DTT and β-mercaptoethanol reduce the inhibitory effect.At every stage of isolating or purifying the proteins, PMSF should be added fresh from the stock solution. PMSF is often not sufficient as the only protease inhibitor in the preparation to protect against decomposition processes, as for example high salt concentration (3.0 M) impairs the inhibitory effect of PMSF.
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Phenylmethyl sulphonyl fluoride, min. 99 %, for biochemistry, 348 mg, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein biology (other), inside Protein biology & Western blot, in Microbiologie.
This shelf is the protein-biology article that is not a membrane and not an extraction kit: stains for gels, buffers, small tools of a protein bench. The method, a gel stain or a quantitation assay, still chooses the bottle.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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