Description
Ponceau S (C.I. 27195), for histology and electrophoresis, Molar mass (M) 760,56 g/mol, CAS No. 6226-79-5, EC No. 228-319-2, Empirical formula C22H12N4Na4O13S4
Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Suitable for rapid control of transfer in Western blot, PVDF membrane should not be completely dried before immunodetection.Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.
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Ponceau S (C.I. 27195), for histology and electrophoresis, 10 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Microscopy stains & staining solutions, inside Dyes, stains & indicators, in Produits chimiques de laboratoire et industriels.
A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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