Description
ROTI®Block, protein free is a ready-to-use, 10× concentrated blocking reagent for blotting, Western blot and ELISA immunodetection workflows. The protein-free composition suits protocols that require blocking without traditional protein blockers, helping reduce background in membrane-based and plate-based assays. It is Physical characteristics include a density of approximately 1,086 g/cm³ and a boiling point of about 100 °C, supporting routine bench handling alongside other immunodetection buffers. ROTI®Block, protein free from manufacturer Carl ROTH is intended for blocking and immunodetection steps where a defined, protein-free blocker is preferred. The 10× format allows flexible preparation of working solutions while limiting storage footprint. Integrate into established Western blot and ELISA procedures, following local laboratory practice for buffer preparation, membrane or plate blocking, and subsequent antibody incubation sequences in cell analysis and protein detection applications.
This article belongs on Protein extraction, purification & markers, in Protein biology & Western blot.
Key features
- Pack sizes: 250 ml, 1 l, 2.5 l, 500 ml
- Density: ~1,086 g/cm³
- Storage temperature: +15 to +25 °C
- Type: Protein-free blocking reagent
- Concentration: 10x concentrated
- Technical Information Sheet:
- Safety Data Sheet:
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Protein biology & Western blot
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Protein extraction, purification & markers
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Questions about ROTI®Block, potein free, ready-to-use, 10x conc., 250 ml, glass
What is this article?
ROTI®Block, potein free, ready-to-use, 10x conc., 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiologie.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiologie.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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