Description
ROTI®CELL RPMI-1640, sterile, with glutamine, with HEPES, ready-to-use, CELLPURE®, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +2 to +8 °C
RPMI-1640 was developed for the cultivation of normal and neoplastic human leukocytes and is based on modified amounts of amino acids and vitamins. RPMI-1640 Medium is very well suited for culturing of a wide range of mammalian suspension cell lines and primary lymphocytes, all leucocytes, B- and T-lymphocytes, as well as myeloma- and hybridoma cells.
Generally, FBS is added to RPMIl media. However, it has been shown that RPMI media also support several cell lines in the absence of serum.
This modification of RPMI contains L-glutamine and 25 mM HEPES, but no pyruvate.
| Use | Cell culture |
| Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 6-8 weeks. |
| Additional info | The detailed composition can be found in the specification. |
Recently viewed
You may also like
Browse related categories
Category
Sub-category
Biological buffers (Tris, HEPES, MOPS)
Catalogue
Produits chimiques de laboratoire et industriels
Brand
Questions about ROTI®CELL RPMI-1640, sterile, with glutamine, with HEPES, 500 ml, plastic
What is this article?
ROTI®CELL RPMI-1640, sterile, with glutamine, with HEPES, 500 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Produits chimiques de laboratoire et industriels.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Produits chimiques de laboratoire et industriels.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.