Description
ROTI®Free Stripping Buffer 2.0, ready-to-use, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C, UN-Nr. 1760
Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
- Effective
- Gentle
- Odour-free
- Simple application w/o heating
- Cost-efficient
Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.
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Questions about ROTI®Free Stripping Buffer 2.0, ready-to-use, 250 ml, glass
What is this article?
ROTI®Free Stripping Buffer 2.0, ready-to-use, 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiologie.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiologie.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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