Description
ROTI®GelStain, 20 000x, ready-to-use, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.
- Green fluorescence
- Alternative for ethidium bromide, non-toxic and non-mutagenic
- For dsDNA, ssDNA and RNA
- Sensitivity comparable to ethidium bromide (approx. 0.3 ng / band)
- For use with common broad-band ethidium bromide photo filters
- Excitation via UV light (302 nm) and blue light (490 nm)
- Compatible with all usual down-stream applications
The dye can be used in a similar manner as ethidium bromide. By adding the dye to the liquid gel, the nucleic acids are stained during the gel run.ROTI®GelStain has a sensitivity of 0.2 ng/band and is thus even slightly more sensitive than ethidium bromide.When bound to nucleic acids in agarose gels, ROTI®GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR® Green foto filters.ROTI®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.
Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light)Emission maximum (bound to DNA): 520 nm
| Max. excitation (DNA-bound) | 302 nm (UV), 490 nm |
| Emissions max. (DNA-bound) | 520 nm |
| Colour | fluorescent/green |
| Sensitivity | 0,2 ng/band |
| ROTH for Future Score - sustainability criteria | • Product features You can find further information on our website - Sustainability criteria at Carl ROTH. |
| ROTH for Future | yes |