Description
ROTI®Prep DNA & RNA Mini, 10 pcs, for molecular biology, Density (D) 1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep DNA & RNA was developed to isolate both DNA and RNA separately from only one sample.The isolation is based on the lysis of a starting sample, the lysate of which is first filtered through a centrifugation column, to the membrane of which the DNA is bound. The filtrate is then transferred to a second centrifugation column, which binds the RNA. The bound DNA and RNA are washed separately in the following steps and collected in separate reaction vessels at the end.
- Simultaneous isolation of DNA and RNA from one sample
- Preparation in the well-established mini-column system
- Fast, easy and reliable
- Extraction time: 15-40 min (depending on the starting material)
- Microbial cell cultures (max. 1x109)
- Fungi and eukaryotic cell cultures (max. 5x106)
- Tissue samples (up to 20 mg)
Lysis Buffer LSR, Washing Buffer WSA (conc.), Washing Buffer WSL (conc.), RNase-free Water, Elution Buffer EB, centrifugation columns DNA (blue), centrifugation columns RNA (violet), collection tubes, elution tubes, detailed instructions.Ethanol 99.8 % (p.a., Art. no. 9065), TE buffer (Art. no. 1052.1) and lysozyme (Art. no. 8259.1) are not included in the kit and should be ordered separately if required.
| Basic material | Microbial cell cultures, fungi and eukaryotic cell cultures, tissue samples |
| Quantity of material | Microbial cell cultures < max. 1x109, eukaryotic cell cultures < 5x106, tissue samples < 20 mg |
| Preparation time | 15-40 min (depending on the source material) |
| Version | Mini prep |
| Automatic use | no |
| Elution volume | DNA: 100 μl; RNA: 30-80 μl ml |
| Typical yield | DNA: 40 μg; RNA: 60 μg |
| Typical purity (A260/A280) | DNA: 1.7-2.0; RNA: 1.8-2.1 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)